Ductus deferens or Vas deferens has a mucosa, muscularis and fibrosa. The mucosa form longitudinal folds and is lined by pseudostratified columnar cells similar to that of ductus epididymis. But the cells are low and stereocilia shows variable distribution, absent form some cells and present in others.
Lamina propria is rich in elastic tissue.
Muscularis is the thickest coat and shows three layers – inner longitudinal, middle circular and outer longitudinal.
The inner muscular layer is thin. The middle circular layer is the thickest and in the stallion, bull and carnivores the fibres of the three layers are interwoven so that the layers are less distinct.
Outer fibrous coat shows blood vessels and nerves.